GST-NEMO (L329P)

NF-κB essential modulator (NEMO) is a regulatory subunit within IκB kinase (IKK). IKK is also composed of two unrelated catalytic subunits called IKKα and IKKβ. NEMO is additionally referred to as IKKγ, IKKAP1, or FIP-3. IKK is responsible for activating the transcription factor, NF-κB, which is involved in immune response. NEMO prefers to bind K63-linked or linear polyubiquitin chains.

The L329P substitution abolishes NEMO interacts with linear polyUb chains and long K63 polyubiquitin chains.

Additional Information

Product Name: GST-NEMO (L329P)
Also Known As: IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma
Catalog No.: I1510
Size: 25 µg
Molecular Weight: 48.2 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~80% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_001099857
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-NEMO (L329P)
Shipping Method: Dry ice shipping
References: 1. Rothwarf DM, et al. (1998) Nature 395, 297 – 300.
2. Yamaoka S, et al. (1998) Cell 93, 1231 – 1240.
3. Ghosh S, Karin M (2002) Cell 109 Suppl, S81 – 96.

Details

NF-κB essential modulator (NEMO) is a regulatory subunit within IκB kinase (IKK). IKK is also composed of two unrelated catalytic subunits called IKKα and IKKβ. NEMO is additionally referred to as IKKγ, IKKAP1, or FIP-3. IKK is responsible for activating the transcription factor, NF-κB, which is involved in immune response. NEMO prefers to bind K63-linked or linear polyubiquitin chains.

The L329P substitution abolishes NEMO interacts with linear polyUb chains and long K63 polyubiquitin chains.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-NEMO (L329P)