Additional Information
| Product Name: | GST-NEMO (L329P) | ||
|---|---|---|---|
| Also Known As: | IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma | ||
| Catalog No.: | I1511 | ||
| Size: | 100 µg | ||
| Molecular Weight: | 48.2 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~80% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_001099857 | ||
| Image(s): | (Click image to enlarge)
|
||
| Shipping Method: | Dry ice shipping | ||
| References: | 1. Rothwarf DM, et al. (1998) Nature 395, 297 – 300. 2. Yamaoka S, et al. (1998) Cell 93, 1231 – 1240. 3. Ghosh S, Karin M (2002) Cell 109 Suppl, S81 – 96. |
Details
NF-κB essential modulator (NEMO) is a regulatory subunit within IκB kinase (IKK). IKK is also composed of two unrelated catalytic subunits called IKKα and IKKβ. NEMO is additionally referred to as IKKγ, IKKAP1, or FIP-3. IKK is responsible for activating the transcription factor, NF-κB, which is involved in immune response. NEMO prefers to bind K63-linked or linear polyubiquitin chains.
The L329P substitution abolishes NEMO interacts with linear polyUb chains and long K63 polyubiquitin chains.
Images:
(Click image to enlarge)
|
| Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified GST-NEMO (L329P) |
