Additional Information
| Product Name: | GST-4xSIM |
|---|---|
| Also Known As: | RNF4-SIM |
| Catalog No.: | I1300 |
| Size: | 100 µg |
| Molecular Weight: | 11.3 kDa |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | 1. Chiariotti L, et al. (1998) Genomics 47(2), 258 – 265. 2. Fedele M, et al. (2000) J Biol Chem 275(11), 7894 – 7901. 3. Hunter T, Sun H (2008) Ernst Schering Found Sump Proc (1), 1 – 16. 4. Tatham MH, et al. (2008) Nat Cell BIol 10(5), 538 – 546. 5. Bruderer R, et al. (2011) EMBO Rep 12(2), 142 – 148. |
Details
The SIM domain contains the four SUMO interaction motifs (SIMs) of RING-finger 4 (RNF4). This SIM domain prefers binding polySUMO chains with four or more SUMOs. It is useful for enrichment of polysumolyated proteins or polySUMO chains.
Images
(Click image to enlarge)
Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-4xSIM
Images
(Click image to enlarge)
Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-4xSIM
