6xHis-ZnF-UBP

Usp5 (IsoT) is a deubiquitinase enzyme that hydrolyzes free polyubiquitin chains. The Usp5 contains two zinc finger domains (ZnF-UBP). This product contains the second ZnF-UBP domain (amino acid 163 – 291). The ZnF-UBP domains bind free ubiquitin chains’ diglycine carboxyl tail, which defines its specificity in binding of free polyubiquitin chains.

Additional Information

Product Name: 6xHis-ZnF-UBP
Also Known As: N/A
Catalog No.: I1411
Size: 500 µg
Molecular Weight: 14.6 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~80% by SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s):

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-ZnF-UBP
Shipping Method: Dry ice shipping
References: 1. Hadari T, et al. (1992) J Biol Chem 267, 719 – 727.
2. Reyes-Turcu FE, et al. (2006) Cell 124, 1197 – 1208.
3. Reyes-Turcu FE, et al. (2008) J Biol Chem 283(28), 19581 – 19592.
4. Avvakumov GV, et al. (2012) Biochemistry 51(6), 1188 – 1198.

Details

Usp5 (IsoT) is a deubiquitinase enzyme that hydrolyzes free polyubiquitin chains. The Usp5 contains two zinc finger domains (ZnF-UBP). This product contains the second ZnF-UBP domain (amino acid 163 – 291). The ZnF-UBP domains bind free ubiquitin chains’ diglycine carboxyl tail, which defines its specificity in binding of free polyubiquitin chains.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-ZnF-UBP