6xHis-TAB2 (NZF)

6xHis was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro pulldown assays and for enrichment of cellular proteins conjugated with lysine 63 polyubiquitin chains in whole cell or tissue lysates. 6xHis-TAB2 (NZF) can be precipitated using Nickel resin. After washing, 6xHis-TAB2 (NZF) and its bound proteins can be eluted by a buffer containing 200 mM imidazole.

Additional Information

Product Name: 6xHis-TAB2 (NZF)
Also Known As: CHTD2; MAP3K7IP2
Catalog No.: I1711
Size: 500 µg
Molecular Weight: 7.7 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~85% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_015093.4
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-TAB2 (NZF)


Shipping Method: Dry ice shipping
References: 1. van Wijk SJ, et al. (2013) Nat Protoc. 8(7):1449-58
2. van Wijk SJ, et al. (2012) Mol Cell. 14;47(5):797-809

Details

6xHis was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro pulldown assays and for enrichment of cellular proteins conjugated with lysine 63 polyubiquitin chains in whole cell or tissue lysates. 6xHis-TAB2 (NZF) can be precipitated using Nickel resin. After washing, 6xHis-TAB2 (NZF) and its bound proteins can be eluted by a buffer containing 200 mM imidazole.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-TAB2 (NZF)