Ubiquitin (S20C)

Ubiquitin (S20C) bears a single amino acid substitution that replaces serine 20 with a cysteine. This substitution has no effect on Ub’s structure and allows label Ub with other groups, such as biotin or fluorescent groups that are conjugated on a thiol reaction group maleimide.

Additional Information

Product Name: Ubiquitin (S20C)
Also Known As: Ub (S20C)
Catalog No.: E1151
Size: 5 mg
Molecular Weight: 8.5 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM HEPES, pH 7.5, 150 mM NaCl, 5 mM TCEP and 10% glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 10 µg purified Ubiquitin (S20C)
Shipping Method: Dry ice shipping
References: No

Details

Ubiquitin (S20C) bears a single amino acid substitution that replaces serine 20 with a cysteine. This substitution has no effect on Ub’s structure and allows label Ub with other groups, such as biotin or fluorescent groups that are conjugated on a thiol reaction group maleimide.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 10 µg purified Ubiquitin (S20C)