Additional Information
Product Name: | N-terminal TAMRA-Ub |
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Also Known As: | TAMRA-Ub |
Catalog No.: | E1161 |
Size: | 1 mg |
Molecular Weight: | 8977 Da |
Species: | Human |
Source: | Chemical synthesis |
Stock: | 20 mM Tris, pH 7.6 at 4 °C, 150 mM NaC and 10% glycerol |
Concentration: | See tube label |
Quality Assurance: | >95% by FPLC. |
Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
PDF Data Sheet: | PDF Datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | See PDF datasheet. |
Shipping Method: | Dry ice shipping |
References: | (1) El Oualid et al. Angew. Chem. Int. Ed. 2010, 49, 10149. (2) de Jong et al. ChemBioChem 2012, 13, 2251. (3) Smit et al. J Biol. Chem 2013, 288, 31728. |
Details
N-terminal TAMRA-Ub (also called TAMRA-Ub) is a fluorescent Ub, in which TAMRA (5-tetramethylrhodamine) is covalently conjugated on the N-terminus of Ub. All seven lysine residues in TARMA-Ub are available for ubiquitination. This product can be used for determination of protein ubiquitination or E1/E2/E3 enzyme activity using sensitive in-gel TAMRA fluorescence. TAMRA fluorescence is detected using excitation/emission wavelengths at 550 nm/590 nm, respectively.
See images in PDF datasheet.