N-terminal TAMRA-Ub

N-terminal TARMA-Ub (also called TARMA-Ub) is a fluorescent Ub, in which TAMRA (5-tetramethylrhodamine) is covalently conjugated on the N-terminus of Ub. All seven lysine residues in TARMA-Ub are available for ubiquitination. This product can be used for determination of protein ubiquitination or E1/E2/E3 enzyme activity using sensitive in-gel fluorescence. TAMRA fluorescence is detected using excitation/emission wavelengths at 550 nm/590 nm, respectively.

Additional Information

Product Name: N-terminal TAMRA-Ub
Also Known As: TAMRA-Ub
Catalog No.: E1160
Size: 100 ug
Molecular Weight: 8977 Da
Species: Human
Source: Chemical synthesis
Stock: 20 mM Tris, pH 7.6 at 4 °C, 150 mM NaC and 10% glycerol
Concentration: See tube label
Quality Assurance: >95% by FPLC.
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): See PDF datasheet.
Shipping Method: Dry ice shipping
References: (1) El Oualid et al. Angew. Chem. Int. Ed. 2010, 49, 10149. (2) de Jong et al. ChemBioChem 2012, 13, 2251. (3) Smit et al. J Biol. Chem 2013, 288, 31728.

Details

N-terminal TAMRA-Ub (also called TAMRA-Ub) is a fluorescent Ub, in which TAMRA (5-tetramethylrhodamine) is covalently conjugated on the N-terminus of Ub. All seven lysine residues in TARMA-Ub are available for ubiquitination. This product can be used for determination of protein ubiquitination or E1/E2/E3 enzyme activity using sensitive in-gel TAMRA fluorescence. TAMRA fluorescence is detected using excitation/emission wavelengths at 550 nm/590 nm, respectively. See images in PDF datasheet.