
Additional Information
Product Name: | N-terminal Biotin-Ubiquitin | ||
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Also Known As: | Biotin-Ub | ||
Catalog No.: | E1141 | ||
Size: | 1 mg | ||
Molecular Weight: | 8.9 kDa | ||
Species: | Human | ||
Source: | Synthetic | ||
Stock: | 20 mM Tris, pH 7.6 at 4 °C, 100 mM NaCl, 10% glycerol | ||
Concentration: | 1 mg/ml in aqueous buffer | ||
Quality Assurance: | > 95% by HPLC | ||
Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
PDF Data Sheet: | PDF Datasheet, MSDS | ||
NCBI RefSeq: | N/A | ||
Image(s): | (Click image to enlarge)
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Shipping Method: | FedEx | ||
References: | N/A |
Details
A single biotin moiety is conjugated on the N-terminus of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The extremely high binding affinity between biotin and streptavidin allows the use of N-terminal biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins; 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody; and 3) setting up high throughput FRET assays to monitor substrate ubiquitination in assays screening E3 ubiquitin ligase inhibitors.
Use
Depending on ubiquitination assay conditions, N-terminal biotin-ubiquitin can used in conjunction with wild type ubiquitin at combined concentration range of 20-50 μM with a 1:4 to 1:20 ratio of N-terminal biotin-Ub: wild type ubiquitin. Images: (Click image to enlarge)
Use
Depending on ubiquitination assay conditions, N-terminal biotin-ubiquitin can used in conjunction with wild type ubiquitin at combined concentration range of 20-50 μM with a 1:4 to 1:20 ratio of N-terminal biotin-Ub: wild type ubiquitin. Images: (Click image to enlarge)
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LC-MS of N-terminal Biotin-Ubiquitin
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