N-terminal Biotin-Ubiquitin

Additional Information

Product Name:

Also Known As:

Biotin-Ub

Catalog No.:

E1141

Size:

1 mg

Molecular Weight:

8.9 kDa

Species:

Human

Source:

Synthetic

Stock:

20 mM Tris, pH 7.6 at 4 °C, 100 mM NaCl, 10% glycerol

Concentration:

1 mg/ml in aqueous buffer

Quality Assurance:

> 95% by HPLC

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet， MSDS

NCBI RefSeq:

N/A

Image(s):

(Click image to enlarge)

LC-MS of N-terminal Biotin-Ubiquitin

Shipping Method:

Dry ice shipping

References:

N/A

Details

A single biotin moiety is conjugated on the N-terminus of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The extremely high binding affinity between biotin and streptavidin allows the use of N-terminal biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins; 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody; and 3) setting up high throughput FRET assays to monitor substrate ubiquitination in assays screening E3 ubiquitin ligase inhibitors.

Use
Depending on ubiquitination assay conditions, N-terminal biotin-ubiquitin can used in conjunction with wild type ubiquitin at combined concentration range of 20-50 μM with a 1:4 to 1:20 ratio of N-terminal biotin-Ub: wild type ubiquitin.

Images:
(Click image to enlarge)

LC-MS of N-terminal Biotin-Ubiquitin