Additional Information
| Product Name: | Fluorescein labeled Ub | ||
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| Also Known As: | No | ||
| Catalog No.: | E1155 | ||
| Size: | 0.2 mg | ||
| Molecular Weight: | 8.5 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, pH 7.6 at 4 °C, 150 mM NaCl, 5 mM TCEP and 10% glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~90% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | N/A | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | No |
Details
Fluorescein labeled Ub is made by reacting Ub(S20C) and fluorescein maleimide. All lysine residues are still available for formation of polyubiquitin chains. It can be used in ubiquitination reactions to generate fluorescein labeled ubiquitinated proteins or fluorescein labeled free polyubiquitin chains.
Images:
(Click image to enlarge)
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A: Coomassie-stained SDS-PAGE of 10 μg fluorescein-Ub B: Visualization of fluorescein-Ub on SDS-PAGE using a gel documentation system excited at 488 nM. Left: normal view; right: inverted view. Lane 1: 80 ng Lane 2: 40 ng Lane 3: 20 ng Lane 4: 10 ng |
