Fluorescein labeled Ub

Fluorescein labeled Ub is made by reacting Ub(S20C) and fluorescein maleimide. All lysine residues are still available for formation of polyubiquitin chains. It can be used in ubiquitination reactions to generate fluorescein labeled ubiquitinated proteins or fluorescein labeled free polyubiquitin chains.

Additional Information

Product Name: Fluorescein labeled Ub
Also Known As: No
Catalog No.: E1156
Size: 1 mg
Molecular Weight: 8.5 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, pH 7.6 at 4 °C, 150 mM NaCl, 5 mM TCEP and 10% glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

A: Coomassie-stained SDS-PAGE of 10 μg fluorescein-Ub
B: Visualization of fluorescein-Ub on SDS-PAGE using a gel documentation system excited at 488 nM.
Lane 1: 6.4 μg
Lane 2: 3.2 μg
Lane 3: 1.6 μg
Lane 4: 0.8 μg
Lane 5: 0.4 μg
Lane 6: 0.2 μg
Lane 7: 0.1 μg
Shipping Method: Dry ice shipping
References: No

Details

Fluorescein labeled Ub is made by reacting Ub(S20C) and fluorescein maleimide. All lysine residues are still available for formation of polyubiquitin chains. It can be used in ubiquitination reactions to generate fluorescein labeled ubiquitinated proteins or fluorescein labeled free polyubiquitin chains.

Images:
(Click image to enlarge)

A: Coomassie-stained SDS-PAGE of 10 μg fluorescein-Ub
B: Visualization of fluorescein-Ub on SDS-PAGE using a gel documentation system excited at 488 nM.
Lane 1: 6.4 μg
Lane 2: 3.2 μg
Lane 3: 1.6 μg
Lane 4: 0.8 μg
Lane 5: 0.4 μg
Lane 6: 0.2 μg
Lane 7: 0.1 μg