Additional Information
| Product Name: | 6xHis-Ubiquitin (L73P) |
|---|---|
| Also Known As: | 6xHis-Ub (L73P) |
| Catalog No.: | E1940 |
| Size: | 1 mg |
| Molecular Weight: | 8.5 kDa |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | >95% by SDS-PAGE |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | 1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786. 2. Pickart CM (1997) FASEB 11(13), 1055 – 1066. 3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479. 4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48. 5. Bremm A, et al. (2012) Methods Mol Biol 832, 291 – 228. |
Details
Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM.
Studies have shown that polyUb chains formed by the Ub(L73P) mutant are resistant to deubiquitination catalyzed by many deubiquitination enzymes.
Images
(Click image to enlarge)
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ubiquitin (L73P) Lane 3: 5 µg purified Ubiquitin (L73P)
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ubiquitin (L73P) Lane 3: 5 µg purified Ubiquitin (L73P)
