6xHis-Ubiquitin (K29R)

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K29R), lysine 29 was substituted with an arginine, thereby it cannot form lysine 29-linked polyubiquitin chains.

Additional Information

Product Name: 6xHis-Ubiquitin (K29R)
Also Known As: 6xHis-UbK29R
Catalog No.: E1750
Size: 1 mg
Molecular Weight: 8.5 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Dry ice shipping
References: 1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786.
2. Pickart CM (1997) FASEB 11(13), 1055 – 1066.
3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.
4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.
5. Bremm A, et al. (2012) Methods Mol Biol 832, 291 – 228.

Details

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K29R), lysine 29 was substituted with an arginine, thereby it cannot form lysine 29-linked polyubiquitin chains.

Images
(Click image to enlarge)


Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ubiquitin (K0)
Lane 3: 5 µg purified 6xHis-Ubiquitin (K6R)
Lane 4: 5 µg purified 6xHis-Ubiquitin (K11R)
Lane 5: 5 µg purified 6xHis-Ubiquitin (K27R)
Lane 6: 5 µg purified 6xHis-Ubiquitin (K29R)
Lane 7: 5 µg purified 6xHis-Ubiquitin (K33R)
Lane 8: 5 µg purified 6xHis-Ubiquitin (K48R)
Lane 9: 5 µg purified 6xHis-Ubiquitin (K63R)