6xHis-Ubiquitin (K11 only)

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K11 only), all lysine residues but lysine 11 were substituted with arginines, thereby it only supports the formation of lysine 11-linked polyubiquitin chains.

Additional Information

Product Name: 6xHis-Ubiquitin (K11 only)
Also Known As: 6xHis-[K11-only]Ub
Catalog No.: E1830
Size: 1 mg
Molecular Weight: 8.5 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Dry ice shipping
References: 1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786.
2. Pickart CM (1997) FASEB 11(13), 1055 – 1066.
3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.
4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.
5. Bremm A, et al. (2012) Methods Mol Biol 832, 291 – 228.

Details

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K11 only), all lysine residues but lysine 11 were substituted with arginines, thereby it only supports the formation of lysine 11-linked polyubiquitin chains.

Images
(Click image to enlarge)


Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ubiquitin (K0)
Lane 3: 5 µg purified 6xHis-Ubiquitin (K6 only)
Lane 4: 5 µg purified 6xHis-Ubiquitin (K11 only)
Lane 5: 5 µg purified 6xHis-Ubiquitin (K27 only)
Lane 6: 5 µg purified 6xHis-Ubiquitin (K29 only)
Lane 7: 5 µg purified 6xHis-Ubiquitin (K33 only)
Lane 8: 5 µg purified 6xHis-Ubiquitin (K48 only)
Lane 9: 5 µg purified 6xHis-Ubiquitin (K63 only)