6xHis-mISG15-GFP

Interferon – stimulated gene 15 (Isg15) is a 15 kDa Ub – like protein. It is linked to proteins in much the same way as Ub, using the E1, E2, E3 cascade. U bE1L and UbcH8 are the specific E1 and E2, respectively, to mediate protein ISGylation. Several E3s have also been found. Isg15 is induced by type I interferons (IFNα/β) and bacterial and viral infections. Studies are still being conducted to discover the biological functions of Isg15. mISG15-GFP is a fusion protein with mouse ISG15 fused at the N-terminus of GFP. It was expressed and purified from E. coli.

Additional Information

Product Name: 6xHis-mISG15-GFP
Also Known As: G1P2; IP17; UCRP; IFI15; hUCRP
Catalog No.: E2200
Size: 25 µg
Molecular Weight: 44.9 kDa
Species: Mouse
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_005101
Image(s): No
Shipping Method: Dry ice shipping
References: 1. D’Cunha J, et al. (1996) Proc Natl Acad Sci USA 93(1), 211 – 215.
2. D’Cunha J, et al. (1996) J Immunol 157(9), 4100 – 4108.
3. Zhang D, et al. (2011) J Interferon Cytokine Res 21 (1), 119 – 130.

Details

Interferon – stimulated gene 15 (Isg15) is a 15 kDa Ub – like protein. It is linked to proteins in much the same way as Ub, using the E1, E2, E3 cascade. U bE1L and UbcH8 are the specific E1 and E2, respectively, to mediate protein ISGylation. Several E3s have also been found. Isg15 is induced by type I interferons (IFNα/β) and bacterial and viral infections. Studies are still being conducted to discover the biological functions of Isg15. mISG15-GFP is a fusion protein with mouse ISG15 fused at the N-terminus of GFP. It was expressed and purified from E. coli.