Protein A Magnetic Polymer Resin

Protein A magnetic resins contain a recombinant protein A covalently coupled on non-porous magnetic polymer resins. Recombinant protein A only contains the five Fc-binding domains of protein A, that can bind to antibodies from various mammalian species, including human, mouse, rabbit, pig and dog, but not goat. Protein A magnetic resins can be used for isolating antibodies from serum, cell culture supernatants or ascites, and for immunoprecipitation and co-immunoprecipitation of antigens from cell lysates or tissue extracts.

Comparing to agarose-based resins, non-porous magnetic polymer resins have much less non-specific binding, and suitable for rapid isolation and elution using a magnetic stand.

Additional Information

Product Name: Protein A Magnetic Polymer Resin
Catalog No.: P5025-5
Size: 5 ml
Bead (Geometry, size): Non-porous superparamagnetic polymer, ~1 µm
Cross-Linked: Recombinant protein A was covalently coupled on resins.
Ligand: Recombinant protein A
Agarose %: N/A
Binding Capacity: ~50 µg human IgG per mg resin
Volume %: Resuspended in 1X PBS, 0.01% Tween-20, 0.02% Sodium azide (NaN3) at a concentration of 10 mg/ml.
Storage Temperature: 4 °C
Application: Antibody purification, immunoprecipitation, and co-immunoprecipitation.
PDF Data Sheet: Datasheet
User's Manual: User's Guide
Image(s): No
Shipping Method: Wet ice shipping

Details

Protein A magnetic resins contain a recombinant protein A covalently coupled on non-porous magnetic polymer resins. Recombinant protein A only contains the five Fc-binding domains of protein A, that can bind to antibodies from various mammalian species, including human, mouse, rabbit, pig and dog, but not goat. Protein A magnetic resins can be used for isolating antibodies from serum, cell culture supernatants or ascites, and for immunoprecipitation and co-immunoprecipitation of antigens from cell lysates or tissue extracts.
Comparing to agarose-based resins, non-porous magnetic polymer resins have much less non-specific binding, and suitable for rapid isolation and elution using a magnetic stand.