Additional Information
| Product Name: | Ni-NTA XPure Agarose Resin |
|---|---|
| Catalog No.: | P3020-5 |
| Size: | 5 ml |
| Bead (Geometry, size): | 45 - 165 µm |
| Cross-Linked: | Yes |
| Ligand: | Nitilotriacetic acid (NTA) |
| Agarose %: | Highly cross-linked 6% agarose |
| Binding Capacity: | > 40mg 6xHis-tagged protein/ml medium |
| Volume %: | 50% (v/v) aqueous suspension containing 20% Ethanol |
| Storage Temperature: | 2-8 °C |
| Application: | Batch, Gravity, MPLC and FPLC |
| PDF Data Sheet: | PDF Datasheet, MSDS |
| User's Manual: | PDF User’s Guide |
| Image(s): | No |
| Shipping Method: | Wet ice shipping |
Details
Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90µm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions.
The XPure series of resins are made for large scale and fine purification using a high-performace liquid chromatography (HPLC) system.
The XPure series of resins are made for large scale and fine purification using a high-performace liquid chromatography (HPLC) system.
