Ni-NTA XPure Agarose Resin

Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90µm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions.

Additional Information

Product Name: Ni-NTA XPure Agarose Resin
Catalog No.: P3020-25
Size: 25 ml
Bead (Geometry, size): 45 - 165 µm
Cross-Linked: Yes
Ligand: Nitilotriacetic acid (NTA)
Agarose %: Highly cross-linked 6% agarose
Binding Capacity: > 40mg 6xHis-tagged protein/ml medium
Volume %: 50% (v/v) aqueous suspension containing 20% Ethanol
Storage Temperature: 2-8 °C
Application: Batch, Gravity, MPLC and FPLC
PDF Data Sheet: PDF Datasheet, MSDS
User's Manual: PDF User’s Guide
Image(s): No
Shipping Method: Wet ice shipping

Details

Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90µm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions.

The XPure series of resins are made for large scale and fine purification using a high-performace liquid chromatography (HPLC) system.