Additional Information
| Product Name: | Ni-IDA XPure Agarose Resin |
|---|---|
| Catalog No.: | P3010-5 |
| Size: | 5 ml |
| Bead (Geometry, size): | Spherical, fine: ~ 20-50 µm |
| Cross-Linked: | Yes |
| Ligand: | Iminodiacetic acid (IDA) |
| Agarose %: | 6% Agarose |
| Binding Capacity: | > 26 mg/ml gel (Binding capacity will vary for each target protein.) |
| Volume %: | 50% (v/v) aqueous suspension containing 20% Ethanol |
| Storage Temperature: | 2-8 °C |
| Application: | Batch, Gravity, MPLC and FPLC |
| PDF Data Sheet: | Datasheet, MSDS |
| User's Manual: | User’s Guide |
| Image(s): |  |
| Shipping Method: | Wet ice shipping |
Details
Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein with a 4X or 6XHis tag. Nickel XPure Agrose Resin provides the advantages of the Nickel cation with the high flow rates of the highly cross-linked agarose beads. Except for batch purification, this product is specially suitable for large scale His-tagged protein purification using FPLC.
The XPure series of resins are made for large scale and fine purification using a high-performance liquid chromatography (HPLC) system.
Ube2I was purified with Ni-IDA XPure Agarose Resin using a gravity column
Lane 1: Molecular weight marker
Lane 2: Cell pellet after sonication
Lane 3: Supernatant after sonication
Lane 4: Flow through after binding
Lane 5: Wash
Lane 6: Elution 1
Lane 7: Elution 2
Lane 8: Elution 3
Lane 9: Elution 4
Lane 10: Elution 5
Ube2I was purified with Ni-IDA XPure Agarose Resin using a gravity column
Lane 1: Molecular weight marker
Lane 2: Cell pellet after sonication
Lane 3: Supernatant after sonication
Lane 4: Flow through after binding
Lane 5: Wash
Lane 6: Elution 1
Lane 7: Elution 2
Lane 8: Elution 3
Lane 9: Elution 4
Lane 10: Elution 5
