Ni-IDA XPure Agarose Resin

Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein with a 4X or 6XHis tag. Nickel XPure Agrose Resin provides the advantages of the Nickel cation with the high flow rates of the highly cross-linked agarose beads. Except for batch purification, this product is specially suitable for large scale His-tagged protein purification using FPLC.

The XPure series of resins are made for large scale and fine purification using a high-performance liquid chromatography (HPLC) system.

Additional Information

Product Name: Ni-IDA XPure Agarose Resin
Catalog No.: P3010-25
Size: 25 ml
Bead (Geometry, size): Spherical, fine: ~ 20-50 µm
Cross-Linked: Yes
Ligand: Iminodiacetic acid (IDA)
Agarose %: 6% Agarose
Binding Capacity: > 26 mg/ml gel (Binding capacity will vary for each target protein.)
Volume %: 50% (v/v) aqueous suspension containing 20% Ethanol
Storage Temperature: 2-8 °C
Application: Batch, Gravity, MPLC and FPLC
PDF Data Sheet: Datasheet, MSDS
User's Manual: User’s Guide
Image(s):
Shipping Method: Wet ice shipping

Details

Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein with a 4X or 6XHis tag. Nickel XPure Agrose Resin provides the advantages of the Nickel cation with the high flow rates of the highly cross-linked agarose beads. Except for batch purification, this product is specially suitable for large scale His-tagged protein purification using FPLC. The XPure series of resins are made for large scale and fine purification using a high-performance liquid chromatography (HPLC) system.



Ube2I was purified with Ni-IDA XPure Agarose Resin using a gravity column

Lane 1: Molecular weight marker
Lane 2: Cell pellet after sonication
Lane 3: Supernatant after sonication
Lane 4: Flow through after binding
Lane 5: Wash
Lane 6: Elution 1
Lane 7: Elution 2
Lane 8: Elution 3
Lane 9: Elution 4
Lane 10: Elution 5