IPTG Solution (1M)

IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in E. coli. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.

Additional Information

Product Name: IPTG Solution (1M)
Also Known As: isopropyl - β - D - thiogalactopyranoside
Catalog No.: P1011-250
Size: 250 ml
CAS: 367-93-1
Formula: C9H18O5S
Molecular Weight: 238.3
Form: Liquid. Sterilized by filtration through a 0.22 micron filter.
Quality Assurance: >99% by HPLC, dioxane-free
Source: Synthetic
Storage: Store at -20°C upon receiving.
PDF Data Sheet: PDF Datasheet, MSDS
Image(s):
Shipping Method: Wet ice shipping
References: J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125.

Details

IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in E. coli. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.


Expression of GST-UCHL3 in BL21 (DE3) cells was induced by the addition of 25 μM (lane 3), 100 μM (lane 4), 200 μM (lane 5) or 400 μM (lane 6) IPTG at 37 0C for 2 hours. Lane 2 without IPTG.