Additional Information
Product Name: | IPTG Solution (1M) |
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Also Known As: | isopropyl - β - D - thiogalactopyranoside |
Catalog No.: | P1011-10 |
Size: | 10 ml |
CAS: | 367-93-1 |
Formula: | C9H18O5S |
Molecular Weight: | 238.3 |
Form: | Liquid. Sterilized by filtration through a 0.22 micron filter. |
Quality Assurance: | >99% by HPLC, dioxane-free |
Source: | Synthetic |
Storage: | Store at -20°C upon receiving. |
PDF Data Sheet: | PDF Datasheet, MSDS |
Image(s): | |
Shipping Method: | Wet ice shipping |
References: | J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125. |
Details
IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in E. coli. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.
Expression of GST-UCHL3 in BL21 (DE3) cells was induced by the addition of 25 μM (lane 3), 100 μM (lane 4), 200 μM (lane 5) or 400 μM (lane 6) IPTG at 37 0C for 2 hours. Lane 2 without IPTG.