Glutathione Xpure Agarose Resin

Glutathione Agarose Resin consists of glutathione covalently bound to agarose beads. This resin has high binding affinity and specificity to the Glutathione S-transferase (GST) protein. GST-tagged proteins can be highly purified by batch or column purification (low flow rate) in a one step purification method. Bound GST-tagged proteins are easily eluted from the resin with buffers containing reduced glutathione.

Additional Information

Product Name: Glutathione Xpure Agarose Resin
Catalog No.: P3060-10
Size: 10 ml
Bead (Geometry, size): Spherical, Standard: ~ 45-165 µm
Cross-Linked: No
Ligand: Glutathione
Agarose %: highly cross-linked 4% Agarose
Binding Capacity: > 10 mg recombinant GST/ml gel (binding capacity may vary for each GST-tagged protein)
Volume %: 50% (v/v) aqueous suspension containing 20% Ethanol
Storage Temperature: 4-8 °C
Application: Batch, FPLC
PDF Data Sheet: DatasheetMSDS
User's Manual: User’s Guide
Image(s):
Shipping Method: Wet ice shipping

Details

Glutathione Agarose Resin consists of glutathione covalently bound to agarose beads. This resin has high binding affinity and specificity to the Glutathione S-transferase (GST) protein. GST-tagged proteins can be highly purified by batch or column purification (low flow rate) in a one step purification method. Bound GST-tagged proteins are easily eluted from the resin with buffers containing reduced glutathione.



GST-E2-25K was purified with Glutathione Agarose Resin using a gravity column.

Lane 1: Molecular weight marker
Lane 2: Cell pellet after sonication
Lane 3: Supernatant after sonication
Lane 4: Flow through after binding
Lane 5: Wash
Lane 6: Elution 1
Lane 7: Elution 2
Lane 8: Elution 3
Lane 9: Elution 4
Lane 10: Elution 5