2-Iodoacetamide

Iodoacetamide is an alkylation agent by reacting with histidine, methionine and sulfhydryl groups of proteins. It is an irreversible inhibitor of all cysteine proteases by alkylation of the catalytic cysteine residue, including deubiquitinating enzymes (DUBs), Cathepsin B, and beta-Amylase. It also inhibits ribonuclease by reacting with histidine residues (occurring at a much slower rate than cysteine). Under limiting quantities and a slightly alkaline pH, Iodoacetamide exclusively reacts with cysteines in proteins.

Solubility: Up to 0.5M in water

Additional Information

Product Name: 2-Iodoacetamide
Also Known As: Iodoacetamide
Catalog No.: P1052-5
Size: 5 g
CAS: 144-48-9
Formula: C2H4INO
Molecular Weight: 184.96 g/mol
Form: Powder
Quality Assurance: >98% by HPLC and NMR
Source: Synthetic
Storage: Eligible for room temperature shipping. Store at -20°C upon receiving
PDF Data Sheet: Download PDF datasheet, MSDS
Image(s): N/A
Shipping Method: Room temperature shipping
References: 1. Benesch, R. and Benesch, R.E., Biochim. Biophys. Acta, 23, 643 (1957).
2. Gurd, F.R.N., Methods Enzymol., 25, 424 (1972).

Details

Iodoacetamide is an alkylation agent by reacting with histidine, methionine and sulfhydryl groups of proteins. It is an irreversible inhibitor of all cysteine proteases by alkylation of the catalytic cysteine residue, including deubiquitinating enzymes (DUBs), Cathepsin B, and beta-Amylase. It also inhibits ribonuclease by reacting with histidine residues (occurring at a much slower rate than cysteine). Under limiting quantities and a slightly alkaline pH, Iodoacetamide exclusively reacts with cysteines in proteins.
Solubility: Up to 0.5M in water