Immunoproteasome Activity Fluorometric Assay Kit I

Immunoproteasome Activity Fluorometric Assay Kit I was designed for assaying the immunoproteasomes' chymotrypsin-like activity in vitro using purified proteasome, cell lysates or tissue extracts. The proteasome cleaves Ac-ANW-AMC and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.


The supplied Ac-ANW-AMC is sufficient for 250 X 100 µl reactions.

Additional Information

Product Name: Immunoproteasome Activity Fluorometric Assay Kit I
Also Known As: N/A
Catalog No.: J4160
Size: 1 Kit
Molecular Weight: N/A
Species: N/A
Source: N/A
Stock: N/A
Concentration: See tube label
Quality Assurance: Inhibitor and substrate > 95% by HPLC and NMR; activity assay using cell or tissue extracts.
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet, protocol
NCBI RefSeq: N/A
Image(s): J4160 Image:
(Click image to enlarge)

AMC fluorescence recorded in reactions containing 50 µg mouse spleen extracts, 50 µM Ac-ANW-AMC in the presence or the absence of 100 µM MG132.

Shipping Method: Dry ice shipping
References: N/A

Details

The supplied Ac-ANW-AMC is sufficient for 250 X 100 µl reactions.

Description

Immunoproteasome Activity Fluorometric Assay Kit I was designed for assaying the immunoproteasomes' chymotrypsin-like activity in vitro using purified proteasome, cell lysates or tissue extracts. The proteasome cleaves Ac-ANW-AMC and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.

Components

• AMC (0.1mM in DMSO)................................................30 µl
• 1000X Ac-ANW-AMC (50 mM in DMSO)......................25 µl
• Mouse spleen extracts (5 mg/ml)..................................100 µl
• MG-132..........................................................................1 mg
• DMSO............................................................................200 µl
• ATP (0.5 M)....................................................................100 µl
• MgCl2 (1 M)...............................................250 µl
• 20X Proteasome Assay Buffer........................................1.35 ml