Human 26S proteasome

The 26S proteasome is an approximately 2.5 mDa large complex composed of the 20S proteasome and the 19S regulatory particle (also called PA700 in mammals). The 20S proteasome has 28 subunits that form a barrel – shaped structure arranged as four heptomeric ring of αββα. Three β subunits have peptidase activities that hydrolyze proteins. Either one or both ends of the 20S proteasome can associate with PA700 to form the 26S proteasome. PA700 contains 19 different proteins that have the ability to bind, deubiquitinate and unfold polyubiquitinated proteins with the consumption of ATP hydrolysis. The 26S proteasome degrades polyubiquitinated proteins, which plays essential roles in regulating various cellular events including protein quality control, gene transcription and signal transduction.

Additional Information

Product Name: Human 26S proteasome
Also Known As: 26S proteasome
Catalog No.: A1101
Size: 50 µg
Molecular Weight: ~3000 kDa
Species: Human
Source: HEK 293
Stock: 20 mM Tris, pH 7.6, 40 mM NaCl, 2 mM βME, 2 mM ATP, 5 mM MgCl2, 10% Glycerol
Concentration: See tube label
Quality Assurance: > 90% by native-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)



A. Coomassie-stained SDS-PAGE of 5 ug purified human 26S proteasome

B. Native-PAGE analysis of 3 ug purified human 26S proteasome.
(1) Overlay assay using SUC-LLVY-AMC to determine the chymotrypsin-like activity of the proteasome.
(2) Coomassie-stained native-PAGE after the overlay assay.
Shipping Method: Dry ice shipping
References: 1. Wang X, et al. (2007) Biochemistry 46, 3553-65.

Details

The 26S proteasome is an approximately 2.5 mDa large complex composed of the 20S proteasome and the 19S regulatory particle (also called PA700 in mammals). The 20S proteasome has 28 subunits that form a barrel – shaped structure arranged as four heptomeric ring of αββα. Three β subunits have peptidase activities that hydrolyze proteins. Either one or both ends of the 20S proteasome can associate with PA700 to form the 26S proteasome. PA700 contains 19 different proteins that have the ability to bind, deubiquitinate and unfold polyubiquitinated proteins with the consumption of ATP hydrolysis. The 26S proteasome degrades polyubiquitinated proteins, which plays essential roles in regulating various cellular events including protein quality control, gene transcription and signal transduction.

Images
(Click image to enlarge)



A. Coomassie-stained SDS-PAGE of 5 ug purified human 26S proteasome

B. Native-PAGE analysis of 3 ug purified human 26S proteasome.
(1) Overlay assay using SUC-LLVY-AMC to determine the chymotrypsin-like activity of the proteasome.
(2) Coomassie-stained native-PAGE after the overlay assay.