Bovine immuno 20S proteasome - Proteasomes / Activators / Subunits

Additional Information

Product Name:

Bovine immuno 20S proteasome

Also Known As:

Immuno 20S proteasome

Catalog No.:

A1500

Size:

25 µg

Molecular Weight:

700 kDa

Species:

Bovine

Source:

Bovine red blood cells

Stock:

20 mM Tris, 20 mM NaCl, 1 mM EDTA, 5 mM βME, 10% Glycerol

Concentration:

See tube label

Quality Assurance:

~95% by native-PAGE, see datasheet for gel image

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet， MSDS

NCBI RefSeq:

N/A

Image(s):

(Click image to enlarge)


Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified Bovine immuno 20S proteasome	Coomassie-stained native-PAGE Lane 1: 5 µg purified Bovine immuno 20S proteasome


Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.	Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.	Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28γ (Cat. # A2300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Shipping Method:

Dry ice shipping

References:

1. Goldberg AL, et al. (1992) Nature 357(6377), 375 – 379.
2. Aki M, et al. (1994) J Biochem 115(2), 257 – 269.
3. Tanaka K (1994) J Leukoc Biol 56(5), 571 – 575.
4. Zaiss DM, et al. (2011) J Immunol 187(5), 2302 – 2309.

Details

Upon stimulation with IFN – γ, the expression of the three catalytic β subunits β1, β2, and β5 with iso-forms β1i (LMP2), β2i (MECL – 1), and β5i (LMP7) are induced, respectively. These subunits are incorporated into the 20S proteasome to form the immune 20S proteasome. It was reported that the immunoproteasome has altered proteolytic activities compared to its normal form, which favor the generation of immunopeptides for antigen presentation.

Images:
(Click image to enlarge)


Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified Bovine immuno 20S proteasome	Coomassie-stained native-PAGE Lane 1: 5 µg purified Bovine immuno 20S proteasome


Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.	Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.	Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28γ (Cat. # A2300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.