Bovine immuno 20S proteasome

Upon stimulation with IFN – γ, the expression of the three catalytic β subunits β1, β2, and β5 with iso-forms β1i (LMP2), β2i (MECL – 1), and β5i (LMP7) are induced, respectively. These subunits are incorporated into the 20S proteasome to form the immune 20S proteasome. It was reported that the immunoproteasome has altered proteolytic activities compared to its normal form, which favor the generation of immunopeptides for antigen presentation.

Additional Information

Product Name: Bovine immuno 20S proteasome
Also Known As: Immuno 20S proteasome
Catalog No.: A1501
Size: 50 µg
Molecular Weight: 700 kDa
Species: Bovine
Source: Bovine red blood cells
Stock: 20 mM Tris, 20 mM NaCl, 1 mM EDTA, 5 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by native-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified Bovine immuno 20S proteasome
Coomassie-stained native-PAGE
Lane 1: 5 µg purified Bovine immuno 20S proteasome



Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively. Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively. Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28γ (Cat. # A2300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.
Shipping Method: Dry ice shipping
References: 1. Goldberg AL, et al. (1992) Nature 357(6377), 375 – 379.
2. Aki M, et al. (1994) J Biochem 115(2), 257 – 269.
3. Tanaka K (1994) J Leukoc Biol 56(5), 571 – 575.
4. Zaiss DM, et al. (2011) J Immunol 187(5), 2302 – 2309.

Details

Upon stimulation with IFN – γ, the expression of the three catalytic β subunits β1, β2, and β5 with iso-forms β1i (LMP2), β2i (MECL – 1), and β5i (LMP7) are induced, respectively. These subunits are incorporated into the 20S proteasome to form the immune 20S proteasome. It was reported that the immunoproteasome has altered proteolytic activities compared to its normal form, which favor the generation of immunopeptides for antigen presentation.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified Bovine immuno 20S proteasome
Coomassie-stained native-PAGE
Lane 1: 5 µg purified Bovine immuno 20S proteasome



Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively. Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively. Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28γ (Cat. # A2300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.