Bovine 26S proteasome

The 26S proteasome is an approximately 2.5 mDa large complex composed of the 20S proteasome and the 19S regulatory particle (also called PA700 in mammals). The 20S proteasome has 28 subunits that form a barrel – shaped structure arranged as four heptomeric ring of αββα. Three β subunits have peptidase activities that hydrolyze proteins. Either one or both ends of the 20S proteasome can associate with PA700 to form the 26S proteasome. PA700 contains 19 different proteins that have the ability to bind, deubiquitinate and unfold polyubiquitinated proteins with the consumption of ATP hydrolysis. The 26S proteasome degrades polyubiquitinated proteins, which plays essential roles in regulating various cellular events including protein quality control, gene transcription and signal transduction. 26S proteasome

Additional Information

Product Name: Bovine 26S proteasome
Also Known As: 26S proteasome
Catalog No.: A1201
Size: 50 µg
Molecular Weight: ~2500 kDa
Species: Bovine
Source: Bovine red blood cells
Stock: 20 mM Tris, pH 7.6, 40 mM NaCl, 2 mM βME, 2 mM ATP, 5 mM MgCl2, 10% Glycerol
Concentration: See tube label
Quality Assurance: At least 90% by native-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)


Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified Bovine 26S proteasome


3 µg purified bovine 26S proteasome was resolved by 4% native-PAGE. The protein was visualized by Coomassie-staining (lane 1); or the proteasome activity was determined by an overlay assay using Suc-LLVY-AMC as the substrate (lane 2).
Shipping Method: Dry ice shipping
References: 1. Waxman L, et al. (1987) J Biol Chem 262(6), 2451 – 2457.
2. Pickart CM (1997) FASEB J 11(13), 1055 – 1066.
3. Xie Y (2010) J Mol Cell Biol 2(6), 308 – 317.

Details

The 26S proteasome is an approximately 2.5 mDa large complex composed of the 20S proteasome and the 19S regulatory particle (also called PA700 in mammals). The 20S proteasome has 28 subunits that form a barrel – shaped structure arranged as four heptomeric ring of αββα. Three β subunits have peptidase activities that hydrolyze proteins. Either one or both ends of the 20S proteasome can associate with PA700 to form the 26S proteasome. PA700 contains 19 different proteins that have the ability to bind, deubiquitinate and unfold polyubiquitinated proteins with the consumption of ATP hydrolysis. The 26S proteasome degrades polyubiquitinated proteins, which plays essential roles in regulating various cellular events including protein quality control, gene transcription and signal transduction.

Images
(Click image to enlarge)


Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified Bovine 26S proteasome


3 µg purified bovine 26S proteasome was resolved by 4% native-PAGE. The protein was visualized by Coomassie-staining (lane 1); or the proteasome activity was determined by an overlay assay using Suc-LLVY-AMC as the substrate (lane 2).