Additional Information
Product Name: | Bovine 20S proteasome |
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Also Known As: | 20S proteasome |
Catalog No.: | A1400 |
Size: | 25 µg |
Molecular Weight: | 700 kDa |
Species: | Bovine |
Source: | Bovine red blood cells |
Stock: | 20 mM Tris, 20 mM NaCl, 1 mM EDTA, 5 mM βME, 10% Glycerol |
Concentration: | See tube label |
Quality Assurance: | ~95% by native-PAGE, see datasheet for gel image |
Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
PDF Data Sheet: | PDF Datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | |
Shipping Method: | Dry ice shipping |
References: | 1. Waxman L, et al. (1987) J Biol Chem 262(6), 2451 – 2457. 2. Ganoth D, et al. (1988) J Biol Chem 263(25), 12412 – 12419. 3. Coux O, et al. (1996) Annu Rev Biochem 65, 801 – 847. 4. Kim HM, et al. (2011) Biochimica Biophysica Acta 1809(2), 67 – 79. |
Details
The 20S proteasome has a barrel – shaped structure arranged as four heptomeric rings of αββα. In eukaryotes, each of α and β ring is composed of seven different proteins. The β1, β2 and β5 subunits have ‘caspase-like’, ‘trypsin-like’ and ‘chymotypsin-like’ activities, respectively. In 26S proteasome-mediated protein degradation, to entry the β chamber of the 20S proteasome that houses the proteolytic sites, a substrate protein has to pass through a substrate translocation channel consisting of the double-ring formed by six ATPases of PA700 and the α chamber formed by α subunits of the 20S proteasome.
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