Additional Information
| Product Name: | Bovine 19S (PA700) | ||||||||
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| Also Known As: | PA700 | ||||||||
| Catalog No.: | A1301 | ||||||||
| Size: | 50 µg | ||||||||
| Molecular Weight: | 900 kDa | ||||||||
| Species: | Bovine | ||||||||
| Source: | Bovine red blood cells | ||||||||
| Stock: | 20 mM Tris, 20 mM NaCl, I mM EDTA, 5 mM βME, 10% Glycerol | ||||||||
| Concentration: | See tube label | ||||||||
| Quality Assurance: | At least 90% by native-PAGE, see datasheet for gel image | ||||||||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||||||||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||||||||
| NCBI RefSeq: | N/A | ||||||||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||||||||
| References: | 1. Waxman L, et al. (1987) J Biol Chem 262(6), 2451 – 2457. 2. Kanayama HO, et al. (1992) Eur J Biochem 206(2), 567 – 578. 3. Glickman MH, et al. (1998) Cell 94, 615 – 623. 4. Da Fonseca PC, et al. (2008) J Biol Chem 283, 23305 – 23314. 5. Kim HM, et al. (2011) Biochim Biophysica Acta 1809(2), 67 – 79. |
Details
The 19S regulatory particle (PA700) locates on either one or both ends of the 20S core particle of the 26S proteasome. It has 19 subunits including six AAA ATPases that associate with α subunits of the 20S proteasome to form the 26S proteasome. PA700 plays an essential role in preparing substrate proteins to be degraded by the 20S proteasome, including binding, deubiquitinating and unfolding polyubiquitinated proteins.
Images
(Click image to enlarge)
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| Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified Bovine 19S (PA700) |
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| Coomassie-stained native-PAGE Lane 1: 5 µg purified Bovine 19S (PA700) |
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| Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA700, the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively. |
