Bovine 19S (PA700)

The 19S regulatory particle (PA700) locates on either one or both ends of the 20S core particle of the 26S proteasome. It has 19 subunits including six AAA ATPases that associate with α subunits of the 20S proteasome to form the 26S proteasome. PA700 plays an essential role in preparing substrate proteins to be degraded by the 20S proteasome, including binding, deubiquitinating and unfolding polyubiquitinated proteins.

Additional Information

Product Name: Bovine 19S (PA700)
Also Known As: PA700
Catalog No.: A1301
Size: 50 µg
Molecular Weight: 900 kDa
Species: Bovine
Source: Bovine red blood cells
Stock: 20 mM Tris, 20 mM NaCl, I mM EDTA, 5 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: At least 90% by native-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified Bovine 19S (PA700)

Coomassie-stained native-PAGE
Lane 1: 5 µg purified Bovine 19S (PA700)

Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA700, the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.
Shipping Method: Dry ice shipping
References: 1. Waxman L, et al. (1987) J Biol Chem 262(6), 2451 – 2457.
2. Kanayama HO, et al. (1992) Eur J Biochem 206(2), 567 – 578.
3. Glickman MH, et al. (1998) Cell 94, 615 – 623.
4. Da Fonseca PC, et al. (2008) J Biol Chem 283, 23305 – 23314.
5. Kim HM, et al. (2011) Biochim Biophysica Acta 1809(2), 67 – 79.

Details

The 19S regulatory particle (PA700) locates on either one or both ends of the 20S core particle of the 26S proteasome. It has 19 subunits including six AAA ATPases that associate with α subunits of the 20S proteasome to form the 26S proteasome. PA700 plays an essential role in preparing substrate proteins to be degraded by the 20S proteasome, including binding, deubiquitinating and unfolding polyubiquitinated proteins.

Images
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified Bovine 19S (PA700)

Coomassie-stained native-PAGE
Lane 1: 5 µg purified Bovine 19S (PA700)

Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA700, the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.