Additional Information
Product Name: | Anti-20S Proteasome |
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Also Known As: | Anti-20S Proteasome Rabbit Polyclonal Antibody |
Catalog No.: | Y2010 |
Size: | 25 µg |
Molecular Weight: | ~55/25 kDa |
Species: | Bovine |
Source: | Bovine 20S proteasome |
Stock: | 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
Concentration: | 2 µg/µl |
Quality Assurance: | Guaranteed for detecting endogenous 20S proteasome in 20 µg cell or tissue lysates |
Storage: | Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
PDF Data Sheet: | PDF Datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | (Click image to enlarge)
1:1000 dilution of the Anti-20S Proteasome antibody for detecting 10 µg (lane 1) and 20 µg (lane 2) of whole HeLa cell lysates. Asterisks mark non-specific bands. |
Shipping Method: | Wet ice shipping |
References: | N/A |
Details
Specificity
Recognizes human, bovine and mouse 20S proteasome; other species has not been tested.
Purification
Protein A affinity chromatography
Applications
WB
Working Conditions
WB (with ECL): 1:1000-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test
Images
(Click image to enlarge)
1:1000 dilution of the Anti-20S Proteasome antibody for detecting 10 µg (lane 1) and 20 µg (lane 2) of whole HeLa cell lysates. Asterisks mark non-specific bands.
1:1000 dilution of the Anti-20S Proteasome antibody for detecting 10 µg (lane 1) and 20 µg (lane 2) of whole HeLa cell lysates. Asterisks mark non-specific bands.