6xHis-PA28gamma

PA28 (also called the 11S regulatory complex) is another activator of the 20S proteasome, which assembles on either one or both ends of the 20S proteasome in an ATP-independent manner. PA28 can greatly enhance the peptidase activities of the 20S proteasome, but not for degradation of ubiquitinated proteins. The PA28 activator is a complex of two alternating homologous subunits, PA28α and PA28β, which assembles as a hexameric ring with an α3β3 stoichiometry. PA28α and PA28β are induced by interferon. Functionally, PA28 enhances the generation of class I peptides for antigen presentation. The third subunit, PA28γ, was recently identified in the nucleus. Recombinant PA28α and PA28β form a heptomeric ring; whereas PA28γ forms a hexameric ring. All of them can stimulate the peptidase activities of the 20S proteasome.PSME3;Ki; PA28G; REG-GAMMA; PA28-gamma;PA28gamma;PA28 gamma

Additional Information

Product Name: 6xHis-PA28gamma
Also Known As: PSME3;Ki; PA28G; REG-GAMMA; PA28-gamma;PA28gamma;PA28 gamma
Catalog No.: A2300
Size: 25 µg
Molecular Weight: 29.5 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_176863
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-PA28gamma

Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28γ (Cat. # A2300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.
Shipping Method: Dry ice shipping
References: 1. Ma CP, et al. (1992) J Biol Chem 267(15), 10515 – 10523.
2. Whitby FG, et al. (2000) Nature 408(6808), 115 – 120.
3. Zhang Z, et al. (2008) EMBO J 27(6), 852 – 864.

Details

PA28 (also called the 11S regulatory complex) is another activator of the 20S proteasome, which assembles on either one or both ends of the 20S proteasome in an ATP-independent manner. PA28 can greatly enhance the peptidase activities of the 20S proteasome, but not for degradation of ubiquitinated proteins. The PA28 activator is a complex of two alternating homologous subunits, PA28α and PA28β, which assembles as a hexameric ring with an α3β3 stoichiometry. PA28α and PA28β are induced by interferon. Functionally, PA28 enhances the generation of class I peptides for antigen presentation. The third subunit, PA28γ, was recently identified in the nucleus. Recombinant PA28α and PA28β form a heptomeric ring; whereas PA28γ forms a hexameric ring. All of them can stimulate the peptidase activities of the 20S proteasome.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-PA28gamma

Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28γ (Cat. # A2300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.