Additional Information
| Product Name: | 6xHis-PA28alpha | |||||
|---|---|---|---|---|---|---|
| Also Known As: | PSME1;PA28A; IFI5111; REGalpha; PA28alpha;PA28-alpha;PA28 alpha | |||||
| Catalog No.: | A2101 | |||||
| Size: | 100 µg | |||||
| Molecular Weight: | 28.7 kDa | |||||
| Species: | Human | |||||
| Source: | Bacterial recombinant | |||||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | |||||
| Concentration: | See tube label | |||||
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image | |||||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | |||||
| PDF Data Sheet: | PDF Datasheet， MSDS | |||||
| NCBI RefSeq: | NM_006263 | |||||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | |||||
| References: | 1. Ma CP, et al. (1992) J Biol Chem 267(15), 10515 – 10523. 2. Whitby FG, et al. (2000) Nature 408(6808), 115 – 120. 3. Cascio P, et al. (2002) The EMO Journal 21, 2636 – 2645. 4. Sijts EJAM, et al. (2011) Cell Mol Life Sci 68(9), 1491 – 1502. |
Details
PA28 (also called the 11S regulatory complex) is another activator of the 20S proteasome, which assembles on either one or both ends of the 20S proteasome in an ATP-independent manner. PA28 can greatly enhance the peptidase activities of the 20S proteasome, but not for degradation of ubiquitinated proteins. The PA28 activator is a complex of two alternating homologous subunits, PA28α and PA28β, which assembles as a hexameric ring with an α3β3 stoichiometry. PA28α and PA28β are induced by interferon. Functionally, PA28 enhances the generation of class I peptides for antigen presentation. The third subunit, PA28γ, was recently identified in the nucleus. Recombinant PA28α and PA28β form a heptomeric ring; whereas PA28γ forms a hexameric ring. All of them can stimulate the peptidase activities of the 20S proteasome.
Images:
(Click image to enlarge)
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| Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-PA28alpha |
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| Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively. |
