6xHis-Adrm1

Adrm1 is an integral Ub receptor of the 26S proteasome. Adrm1 binds both Ub and polyUb chains via its N-terminal Pru domain. The Pru domain also binds S1 to locate Adrm1 on the 26S proteasome. The C-terminal domain of Adrm1 binds the deubiquitinating enzyme Uch37, though which Uch37 is recruited to the 26S proteasome.

Additional Information

Product Name: 6xHis-Adrm1
Also Known As: ADRM1;ARM1; GP110; Rpn13
Catalog No.: A3400
Size: 25 µg
Molecular Weight: 42.2 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_007002
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Adrm1
Shipping Method: Dry ice shipping
References: 1. Verma R, et al. (2000) Mol Biol Cell 11(10), 3425 – 3439.
2. Jorgensen JP, et al. (2006) J Mol Biol 360(5), 1043 – 1052.
3. Husnjak K, et al. (2008) Nature 453, 481 – 488.

Details

Adrm1 is an integral Ub receptor of the 26S proteasome. Adrm1 binds both Ub and polyUb chains via its N-terminal Pru domain. The Pru domain also binds S1 to locate Adrm1 on the 26S proteasome. The C-terminal domain of Adrm1 binds the deubiquitinating enzyme Uch37, though which Uch37 is recruited to the 26S proteasome.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Adrm1