26S Proteasome-mediated Protein Degradation Kit

This kit is designed for assaying 26S proteasome-mediated degradation of ubiquitinated proteins in vitro. It contains highly purified active bovine 26S proteasome that has been used by different research groups for assaying protein degradation.

Additional Information

Product Name: 26S Proteasome-mediated Protein Degradation Kit
Also Known As: N/A
Catalog No.: J4330
Size: 1 Kit
Molecular Weight: N/A
Species: N/A
Source: N/A
Stock: Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: Enzyme >95% by SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet
NCBI RefSeq: N/A
Image(s): N/A
Shipping Method: Dry ice shipping
References: 1. Liu CW, Li X, Thompson D, Wooding K, Chang TL, Tang Z, Yu H, Thomas PJ, DeMartino GN. Mol Cell. 2006;24(1):39-50.
2. Jacobson AD, Zhang NY, Xu P, Han KJ, Noone S, Peng J, Liu CW. J Biol Chem. 2009;284(51):35485-94.
3. Hemantha HP, Bavikar SN, Herman-Bachinsky Y, Haj-Yahya N, Bondalapati S, Ciechanover A, Brik A. J Am Chem Soc. 2014;136(6):2665-73.

Details

* The supplied materials are sufficient for setting up 32 X 25 ul reactions.

Description

This kit is designed for assaying 26S proteasome-mediated degradation of ubiquitinated proteins in vitro. It contains highly purified active bovine 26S proteasome that has been used by different research groups for assaying protein degradation (see references).

Components

• Bovine 26S proteasome (200 nM).............................100 μl
• Epoxomicin (10 mM in DMSO).....................................3 μl
• ATP (500 mM).............................................................20 μl
• 10X Degradation Buffer.............................................150 μl

Notes

1. 10X Degradation Buffer: 400 mM Tris, pH 7.1 at 37°C, 400 mM NaCl, 50 mM MgCl2, 20 mM β-ME.
2. The supplied proteasome-specific inhibitor epoxomicin was dissolved in DMSO at 10 mM, dilute 100 X for use.

References

1. Liu CW, Li X, Thompson D, Wooding K, Chang TL, Tang Z, Yu H, Thomas PJ, DeMartino GN. Mol Cell. 2006;24(1):39-50.
2. Jacobson AD, Zhang NY, Xu P, Han KJ, Noone S, Peng J, Liu CW. J Biol Chem. 2009;284(51):35485-94.
3. Hemantha HP, Bavikar SN, Herman-Bachinsky Y, Haj-Yahya N, Bondalapati S, Ciechanover A, Brik A. J Am Chem Soc. 2014;136(6):2665-73.