Proteasome Activity Fluorometric Assay Kit II

Proteasome Activity Fluorometric Assay Kit II was designed for assaying the constitutive proteasomes' chymotrypsin-like, trypsin-like and caspase-like activities in vitro using purified proteasome, cell lysates or tissue extracts. The proteasome cleaves the supplied fluorogenic substrates, and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.

Additional Information

Product Name: Proteasome Activity Fluorometric Assay Kit II
Also Known As: N/A
Catalog No.: J4120
Size: 1 Kit
Molecular Weight: N/A
Species: N/A
Source: N/A
Stock: N/A
Concentration: See tube label
Quality Assurance: Inhibitor and substrate > 98% by HPLC and NMR; activity assay using cell lysates.
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet, protocol
NCBI RefSeq: N/A
Image(s): J4120 Image:
(Click image to enlarge)

AMC fluorescence recorded in reactions containing 50 µg HEK293T cell lysates, 50 µM Suc-LLVY-AMC, Z-LLE-AMC or Boc-LRR-AMC, in the presence of absence of 100 µM MG132.

Shipping Method: Dry ice shipping
References: N/A

Details

Each supplied fluorogenic substrate is sufficient for 250 x 100 µl reactions.

Description

Proteasome Activity Fluorometric Assay Kit II was designed for assaying the constitutive proteasomes' chymotrypsin-like, trypsin-like and caspase-like activities in vitro using purified proteasome, cell lysates or tissue extracts. The proteasome cleaves the supplied fluorogenic substrates and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.

Suc-LLVY-AMC, Boc-LRR-AMC and Z-LLE-AMC are used to monitor the chymotrypsin-like, trypsin-like and caspase-like activities of the proteasome, respectively.

Components

• AMC (0.1 mM in DMSO)....................................................30 µl
• 1000X SUC-LLVY-AMC (50 mM in DMSO)........................25 µl
• 1000X BOC-LRR-AMC (50 mM in DMSO)........................25 µl
• 1000X Z-LLE-AMC (50 mM in DMSO)...............................25 µl
• HEK293T cell lysates (5mg/ml)..........................................100 µl
• MG-132..............................................................................1 mg
• DMSO................................................................................200 µl
• ATP (0.5M).........................................................................100 µl
• MgCl2 (1M)....................................................250 µl
• 20X Proteasome Assay Buffer........................................3x 1.35 ml