Nocodazole

Nocodazole is a microtubule inhibitor which reversibly binds tubulin and alters tubulin-associated GTP hydrolysis as well as microtubule dynamics. While nocodazole is used to study many aspects on microtubule function, it is most commonly used to synchronize cell cycling in culture. Nocodazole is also used to induce the formation of Golgi ministacks in eukaryotic cells.

Additional Information

Product Name: Nocodazole
Also Known As: N-​[6-​(2-​thienylcarbonyl)-​1H-​benzimidazol-​2-​yl]-​methyl ester-​carbamic acid
Catalog No.: F6120
Size: 10 mg
Molecular Weight: 301.32 Da
Species: N/A
Source: Synthetic
Stock: Powder
Concentration: N/A
Quality Assurance: >99% by HPLC and NMR
Storage: Eligible for room temperature shipping. Store at -20°C upon receiving; protect from air and light
PDF Data Sheet: PDF Datasheet, MSDS
NCBI RefSeq: N/A
Image(s): N/A
Shipping Method: Room temperature shipping
References: 1. Vasquez RJ, et al. (1997) Mol.Biol.Cell 8(6):973-85.
2. Mikhailov A, et al. (1998) Cell.Motil.Cytoskeleton 41(4):325-40.
3. Webb JL, et al. (2004) Int.J.Biochem.Cell Biol. 36(12):2541-50.
4. Mizushima N, et al. (2010) Cell 140(3):313-26

Details

Nocodazole is a microtubule inhibitor which reversibly binds tubulin and alters tubulin-associated GTP hydrolysis as well as microtubule dynamics. While nocodazole is used to study many aspects on microtubule function, it is most commonly used to synchronize cell cycling in culture. Nocodazole is also used to induce the formation of Golgi ministacks in eukaryotic cells.

How to use
Nocodazole was used at 40-100 ng/mL of culture medium for a duration of 12-18 hours in cell synchronization experiments.
For cell synchronization experiments, nocodazole is usually employed at a concentration of 40-100 ng/mL of culture medium for a duration of 12-18 hours. For inducing the formation of Golgi ministacks incubate in 33 μM nocodazole for 3 hours.