MG-132

MG-132 is a reversible proteasome inhibitor that belongs to the family known as peptide aldehydes. MG-132 is cell permeable and can be used to treat various mammalian cells to block proteasome activities. MG-132 can be dissolved in DMSO for stock solution up to 100 mM. The typical concentrations for cell culture use are 1-10 µM. For in vitro use, the typical concentrations are 50-100 µM.

Additional Information

Product Name: MG-132
Also Known As: MG132, Z-LLL-al, Z-Leu-Leu-Leu-CHO, CAS#: 133407-82-6
Catalog No.: F1101
Size: 25 mg
Molecular Weight: 475.6 Da
Species: N/A
Source: Synthetic
Stock: Powder
Concentration: N/A
Quality Assurance: >98% by HPLC and NMR
Storage: Eligible for room temperature shipping. Store at -20°C upon receiving; protect from air and ligh.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Room temperature shipping
References: 1. Wilk S, et al. (1993) Enzyme Protein 47(4 – 6), 306 – 313.
2. Bush DT, et al. (1997) J Biol Chem 272(14), 9086 – 9092.
3. Myung J, et al. (2001 Med Res Rev 21(4), 245 – 273.

Details

MG-132 is a reversible proteasome inhibitor that belongs to the family known as peptide aldehydes. MG-132 is cell permeable and can be used to treat various mammalian cells to block proteasome activities. MG-132 can be dissolved in DMSO for stock solution up to 100 mM. The typical concentrations for cell culture use are 1-10 µM. For in vitro use, the typical concentrations are 50-100 µM.

Images
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100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 µM MG-132 for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 µM SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.


Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM MG132 (lane 2)-treated HEK293T cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. Cells were treated with DMSO or MG-132 for 4 hours.