Additional Information
| Product Name: | MG-132 |
|---|---|
| Also Known As: | MG132, Z-LLL-al, Z-Leu-Leu-Leu-CHO, CAS#: 133407-82-6 |
| Catalog No.: | F1100 |
| Size: | 5 mg |
| Molecular Weight: | 475.6 g/mol |
| Species: | N/A |
| Source: | Synthetic |
| Stock: | Powder |
| Concentration: | N/A |
| Quality Assurance: | >98% by HPLC and NMR |
| Storage: | Eligible for room temperature shipping. Store at -20°C upon receiving; protect from air and ligh |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge) 100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 µM MG-132 for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 µM SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.  Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM MG132 (lane 2)-treated HEK293T cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. Cells were treated with DMSO or MG-132 for 4 hours. |
| Shipping Method: | Room temperature shipping |
| References: | 1. Wilk S, et al. (1993) Enzyme Protein 47(4 – 6), 306 – 313. 2. Bush DT, et al. (1997) J Biol Chem 272(14), 9086 – 9092. 3. Myung J, et al. (2001 Med Res Rev 21(4), 245 – 273. |
Details
MG-132 is a reversible proteasome inhibitor that belongs to the family known as peptide aldehydes. MG-132 is cell permeable and can be used to treat various mammalian cells to block proteasome activities. MG-132 can be dissolved in DMSO for stock solution up to 100 mM. The typical concentrations for cell culture use are 1-10 µM. For in vitro use, the typical concentrations are 50-100 µM.
Images
(Click image to enlarge)
100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 µM MG-132 for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 µM SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.
Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM MG132 (lane 2)-treated HEK293T cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. Cells were treated with DMSO or MG-132 for 4 hours.
Images
(Click image to enlarge)
100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 µM MG-132 for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 µM SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.
Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM MG132 (lane 2)-treated HEK293T cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. Cells were treated with DMSO or MG-132 for 4 hours.
