
Additional Information
Product Name: | IU1 |
---|---|
Also Known As: | 1-[1-(4-Fluorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl]-2-(1-pyrrolidinyl)ethanone; CAS#: 314245-33-5 |
Catalog No.: | F4101 |
Size: | 25 mg |
Molecular Weight: | 300.4 Da |
Species: | N/A |
Source: | Synthetic |
Stock: | Powder |
Concentration: | N/A |
Quality Assurance: | >98% by HPLC and NMR |
Storage: | Eligible for room temperature shipping. Store at -20°C upon receiving; protect from air and ligh |
PDF Data Sheet: | Download PDF datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | No |
Shipping Method: | FedEx |
References: | Lee BH et al. (2010) Nature, 467, 179-184 |
Details
IU1 is a reversible and specific inhibitor of proteasome-associating Usp14. It does not inhibit other deubiquitinating enzymes of the 26S proteasome. It partially inhibits the ubiquitin chain trimming activity of the 26S proteasome, which could function to stimulate the degradation of some proteasomal substrates.
Images
(Click image to enlarge)

Equal amount of whole cell lysates prepared from DMSO (lane 1) or 75 μM IU1 (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. HEK293TCells were treated with DMSO or IU1 for 5 hours.
Images
(Click image to enlarge)

Equal amount of whole cell lysates prepared from DMSO (lane 1) or 75 μM IU1 (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. HEK293TCells were treated with DMSO or IU1 for 5 hours.