Immunoproteasome Activity Fluorometric Assay Kit II

Immunoproteasome Activity Fluorometric Assay Kit II was designed for assaying the immunoproteasomes' chymotrypsin-like, trypsin-like and branched amino acid preferring activities in vitro using purified proteasomes, cell lysates or tissue extracts. The proteasome cleaves these fluorogenic substrates, and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.


Each supplied substrate is sufficient for 250 X 100 µl reactions.

Additional Information

Product Name: Immunoproteasome Activity Fluorometric Assay Kit II
Also Known As: N/A
Catalog No.: J4170
Size: 1 Kit
Molecular Weight: N/A
Species: N/A
Source: N/A
Stock: N/A
Concentration: See tube label
Quality Assurance: Inhibitor and substrate > 95% by HPLC and NMR; activity assay using cell or tissue extracts.
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet, protocol
NCBI RefSeq: N/A
Image(s): J4170 Image:
(Click image to enlarge)

AMC fluorescence recorded in reactions containing 50 µg mouse spleen extracts, 50 µM Ac-ANW-AMC, Ac-PAL-AMC or Ac-KQL-AMC, in the presence or the absence of 100 µM MG132.

Shipping Method: Dry ice shipping
References: N/A

Details

Each supplied substrate is sufficient for 250 X 100 µl reactions.

Description

Immunoproteasome Activity Fluorometric Assay Kit II was designed for assaying the immunoproteasomes' chymotrypsin-like, trypsin-like and branched amino acid preferring activities in vitro using purified proteasome, cell lysates or tissue extracts. The proteasome cleaves these fluorogenic substrates, and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.

Components

• AMC (0.1mM in DMSO)................................................30 µl
• 1000X Ac-ANW-AMC (50 mM in DMSO)......................25 µl
• 1000X Ac-PAL-AMC (50 mM in DMSO)......................25 µl
• 1000X Ac-KQL-AMC (50 mM in DMSO)......................25 µl
• Mouse spleen extracts (5 mg/ml)..................................100 µl
• MG-132..........................................................................1 mg
• DMSO............................................................................200 µl
• ATP (0.5 M)....................................................................100 µl
• MgCl2 (1 M)...............................................250 µl
• 20X Proteasome Assay Buffer........................................3 X 1.35 ml