Additional Information
Product Name: | Epoxomicin |
---|---|
Also Known As: | CAS# 134381-21-8 |
Catalog No.: | F1400-5 |
Size: | 5 mg |
Molecular Weight: | 554.72 g/mol |
Species: | N/A |
Source: | Synthetic |
Stock: | Powder |
Concentration: | N/A |
Quality Assurance: | >98% by HPLC and NMR |
Storage: | Eligible for room temperature shipping. Store at -20°C upon receiving; protect from air and ligh |
PDF Data Sheet: | Download PDF datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | No |
Shipping Method: | Room temperature shipping |
References: | 1. M. Hanada, et al. (1992) J. Antibiot., 45, 1746 2. L. Meng, et al. (1999) PNAS, 96, 10403 3. K.B. Kim, et al. Bioorg. (1999) Med. Chem. Lett., 9, 3335 |
Details
Epoxomicin, originally isolated from Actinomyces sp., is a cell-permeable, potent, selective and irreversible proteasome inhibitor. It inhibits chymotrypsin-like, trypsin-like and caspase-like activities of the proteasome.
Images
(Click image to enlarge)
100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 µM Epoxomicin for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 µM SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.
Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM Epoxomicin (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. HEK293TCells were treated with DMSO or Epoxomicin for 4 hours.
Images
(Click image to enlarge)
100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 µM Epoxomicin for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 µM SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.
Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM Epoxomicin (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. HEK293TCells were treated with DMSO or Epoxomicin for 4 hours.