Epoxomicin

Epoxomicin, originally isolated from Actinomyces sp., is a cell-permeable, potent, selective and irreversible proteasome inhibitor. It inhibits chymotrypsin-like, trypsin-like and caspase-like activities of the proteasome.

Additional Information

Product Name: Epoxomicin
Also Known As: CAS# 134381-21-8
Catalog No.: F1400-5
Size: 5 mg
Molecular Weight: 554.72 g/mol
Species: N/A
Source: Synthetic
Stock: Powder
Concentration: N/A
Quality Assurance: >98% by HPLC and NMR
Storage: Eligible for room temperature shipping. Store at -20°C upon receiving; protect from air and ligh
PDF Data Sheet: Download PDF datasheet, MSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Room temperature shipping
References: 1. M. Hanada, et al. (1992) J. Antibiot., 45, 1746
2. L. Meng, et al. (1999) PNAS, 96, 10403
3. K.B. Kim, et al. Bioorg. (1999) Med. Chem. Lett., 9, 3335

Details

Epoxomicin, originally isolated from Actinomyces sp., is a cell-permeable, potent, selective and irreversible proteasome inhibitor. It inhibits chymotrypsin-like, trypsin-like and caspase-like activities of the proteasome.

Images
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100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 µM Epoxomicin for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 µM SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.


Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM Epoxomicin (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. HEK293TCells were treated with DMSO or Epoxomicin for 4 hours.