b-AP15

b-AP15 is a specific inhibitor of the deubiquitinating enzymes Uch37 and Usp14 of the 26S proteasome. It blocks the deubiquitinating activity of the 26S proteasome. Treating cells with b-AP15 causes the accumulation of polyubiquitinated proteins, inhibition of proteasomal degradation, upregulation of apoptotic markers and cell death.

Additional Information

Product Name: b-AP15
Also Known As: N/A
Catalog No.: F2100
Size: 2 mg
Molecular Weight: 419.4 Da
Species: N/A
Source: Synthetic
Stock: Powder
Concentration: N/A
Quality Assurance: >98% by HPLC and NMR
Storage: Eligible for room temperature shipping. Store at -20°C upon receiving; protect from air and ligh
PDF Data Sheet: Download PDF datasheet, MSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Room temperature shipping
References: 1. D’Acry P, et al. (2011) Nat. Med. 17. 1636.

Details

b-AP15 is a specific inhibitor of the deubiquitinating enzymes Uch37 and Usp14 of the 26S proteasome. It blocks the deubiquitinating activity of the 26S proteasome. Treating cells with b-AP15 causes the accumulation of polyubiquitinated proteins, inhibition of proteasomal degradation, upregulation of apoptotic markers and cell death.

Images
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Equal amount of whole cell lysates prepared from DMSO (lane 1) or 2 μM b-AP15 (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. HEK293TCells were treated with DMSO or b-AP15 for 5 hours.