Additional Information
| Product Name: | b-AP15 |
|---|---|
| Also Known As: | No |
| Catalog No.: | F2101 |
| Size: | 10 mg |
| Molecular Weight: | 419.4 Da |
| Species: | N/A |
| Source: | Synthetic |
| Stock: | Powder |
| Concentration: | N/A |
| Quality Assurance: | >98% by HPLC and NMR |
| Storage: | Eligible for room temperature shipping. Store at -20°C upon receiving; protect from air and ligh |
| PDF Data Sheet: | Download PDF datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Room temperature shipping |
| References: | 1. D’Acry P, et al. (2011) Nat. Med. 17. 1636. |
Details
b-AP15 is a specific inhibitor of the deubiquitinating enzymes Uch37 and Usp14 of the 26S proteasome. It blocks the deubiquitinating activity of the 26S proteasome. Treating cells with b-AP15 causes the accumulation of polyubiquitinated proteins, inhibition of proteasomal degradation, upregulation of apoptotic markers and cell death.
Images
(Click image to enlarge)
Equal amount of whole cell lysates prepared from DMSO (lane 1) or 2 μM b-AP15 (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. HEK293TCells were treated with DMSO or b-AP15 for 5 hours.
Images
(Click image to enlarge)
Equal amount of whole cell lysates prepared from DMSO (lane 1) or 2 μM b-AP15 (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. HEK293TCells were treated with DMSO or b-AP15 for 5 hours.
