Misfolded Protein Ubiquitination Kit

This kit is designated for monitoring CHIP-mediated ubiquitination of user-supplied misfolded proteins. CHIP is an E3 ubiquitin ligase that directly or via Hsp70 interacts with misfolded proteins and promotes their polyubiquitination, which targets misfolded proteins for proteasomal degradation.

Additional Information

Product Name: Misfolded Protein Ubiquitination Kit
Also Known As: N/A
Catalog No.: J5110
Size: 1 Kit
Molecular Weight: N/A
Species: N/A
Source: N/A
Stock: Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: Enzyme >95% by SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet, MSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Dry ice shipping
References: N/A

Details

* This kit is enough for setting up 20 x 20 µl reactions.

Description
This kit is designated for monitoring CHIP-mediated ubiquitination of user-supplied misfolded proteins. CHIP is an E3 ubiquitin ligase that directly or via Hsp70 interacts with misfolded proteins and promotes their polyubiquitination, which targets misfolded proteins for proteasomal degradation.

Components

• 10x Human Ubiquitin (500 uM)..............................................40 µl
• 20x UBE1 (E1, 2 uM))............................................................20 µl
• 10x 6xHis-UbE2D3 (E2, 20 uM)............................................40 µl
• 20x 6XHis-CHIP (E3, 40 uM))................................................20 µl
• 10x Hsp70 (20 uM)................................................................40 µl
• 5x Ubiquitination Buffer.......................................................150 µl
• 10x ATP (40 mM)...................................................................60 µl