Loading Control Antibody Kit

Loading Control Antibody Kit contains three commonly-used loading control antibodies. The anti-GAPDH, beta-actin and beta-tubulin antibodies in this kit are mouse monoclonal antibodies purified by protein A affinity chromatography from mouse ascites fluids. You save approximately 30% compared to buy individually.

Additional Information

Product Name: Loading Control Antibody Kit
Also Known As: N/A
Catalog No.: J5200
Size: 1 Kit
Molecular Weight: ~55/25 kDa
Species: Human
Source: No
Stock: 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)
Concentration: 1 µg/µl
Quality Assurance: Guaranteed for detecting endogenous loading control proteins in 20 µg cell or tissue lysates
Storage: Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Wet ice shipping
References: N/A

Details

Loading Control Antibody Kit contains three commonly-used loading control antibodies. The anti-GAPDH, beta-actin and beta-tubulin antibodies in this kit are mouse monoclonal antibodies purified by protein A affinity chromatography from mouse ascites fluids. You save approximately 30% compared to buy individually.

Components
Anti-GAPDH (Clone# GA1R): 100 µg
Anti-beta-Actin (Clone# BA3R): 100 µg
Anti-beta-Tubulin (Clone# BT7R): 100 µg

Purification
Protein A affinity chromatography from mouse ascites fluid

Applications
Dot, ELISA, IS, WB

Working Conditions
For Western Blot with ECL, Anti-GAPDH 1:1000-10,000 dilution; Anti-beta-Actin 1:1000-5,000 dilution; Anti-beta-Tubulin 1:1000-5,000 dilution.
For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test

Images
(Click image to enlarge)


Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)