Details
The supplied Ac-ANW-AMC is sufficient for 250 X 100 µl reactions.
Description
Immunoproteasome Activity Fluorometric Assay Kit I was designed for assaying the immunoproteasomes' chymotrypsin-like activity in vitro using purified proteasome, cell lysates or tissue extracts. The proteasome cleaves Ac-ANW-AMC and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.
Components
• AMC (0.1mM in DMSO)................................................30 µl
• 1000X Ac-ANW-AMC (50 mM in DMSO)......................25 µl
• Mouse spleen extracts (5 mg/ml)..................................100 µl
• MG-132..........................................................................1 mg
• DMSO............................................................................200 µl
• ATP (0.5 M)....................................................................100 µl
• MgCl2 (1 M)...............................................250 µl
• 20X Proteasome Assay Buffer........................................1.35 ml