Ubiquitin E2 Screening Kit

Description

The ubiquitin E2 Screening Kit (Cat# J1100) contains ubiquitin (Ub)-activating enzyme (UbE1), Ub and 29 Ub-conjugating enzymes (E2s). It can be used for 1) identification of the E2 enzyme paired for a specific Ub ligase (E3) for a protein substrate, and 2) assessing/profiling E2 activity by monitoring the E2-Ub thioester conjugate formation under non-reducing condition.

A few E2s are specific for other Ub-like proteins, including SUMO, Nedd8 and ISG15. If you need to assess SUMO and Nedd8 reactivity, please use the ubiquitin/SUMO/Nedd8 E2 Screening Kit (Cat# J1300) that contains additional SUMO- or Nedd8-activating E1, as well as SUMO2 and Nedd8 proteins.


Additional Information

Product Name: Ubiquitin E2 Screening Kit
Also Known As: E2 screening kit
Catalog No.: J1100
Size: 1 Kit
Molecular Weight: N/A
Species: Human
Source: Bacterial or insect cell recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: 90% - 95% based on SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet, MSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Dry ice shipping
References: N/A

Details

Description
The ubiquitin E2 Screening Kit (Cat# J1100) contains ubiquitin (Ub)-activating enzyme (UbE1), Ub and 29 Ub-conjugating enzymes (E2s). It can be used for 1) identification of the E2 enzyme paired for a specific Ub ligase (E3) for a protein substrate, and 2) assessing/profiling E2 activity by monitoring the E2-Ub thioester conjugate formation under non-reducing condition.
A few E2s are specific for other Ub-like proteins, including SUMO, Nedd8 and ISG15. If you need to assess SUMO and Nedd8 reactivity, please use the ubiquitin/SUMO/Nedd8 E2 Screening Kit (Cat# J1300) that contains additional SUMO or Nedd8-activating E1, as well as SUMO2 and Nedd8 proteins.
Components

• 20X UBE1 (2 µM)......................................................100µl
• 10X E2 Ub-conjugating enzymes
(29 E2s, see table below, 20µM)................................20 µl each
• 10X Human Ubiquitin (500 µM)................................200 µl
• 10X Ubiquitination Buffer.............................................1 ml
• 40 mM ATP...............................................................250 µl

E2 Name

1: 6XHis-UbE2A (Rad6A)
2: 6XHis-UbE2B
3: 6XHis-UbE2C(UbcH10 )
4: 6XHis-UbE2D1 (UbcH5A)
5: 6XHis-UbE2D2 (UbcH5B)
6: 6XHis-UbE2D3 (UbcH5C)
7: 6XHis-UbE2D4 (UbcH5D)
8: 6XHis-UbE2E1 (UbcH6)
9: 6XHis-UbE2E2 (UbcH8)
10: 6XHis-UbE2E3 (UbcH9)
11: 6XHis-UbE2F
12: 6XHis-UbE2G1
13: 6XHis-UbE2G2
14: 6XHis-UbE2H (UbcH2)
15: 6XHis-UbE2I
16: 6XHis-UbE2J1(1-282)
17: GST-UbE2K (E2-25K)
18: 6XHis-UbE2L3
19: 6XHis-UbE2L6
20: 6XHis-UbE2M (UbcH12)
21: 6XHis-UbEN (Ubc13/Uev1a)
22: 6XHis-UbE2Q2
23: 6XHis-UbE2R1 (Cdc34)
24: 6XHis-UbE2R2
25: 6XHis-UbE2S
26: 6XHis-UbE2T
27: 6xHis-Ubc13/UbE2V2
28: 6XHis-UbE2W
29: 6XHis-UbE2Z