E2 Screening Kit

The E2 Screening Kit is developed for identification of the E2 Ub-conjugating enzyme(s) for a specific E3 Ub ligase and/or a protein substrate.

Additional Information

Product Name: E2 Screening Kit
Also Known As: N/A
Catalog No.: J1100
Size: 1 Kit
Molecular Weight: N/A
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: 90% - 95% based on SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet, MSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Dry ice shipping
References: N/A

Details

Description

The E2 Screening Kit is developed for identification of the E2 Ub-conjugating enzyme(s) for a specific E3 Ub ligase and/or a protein substrate.

Components

• 20X UBE1 (2 µM)......................................................100µl
• 10X E2 Ub conjugating enzymes
(29 E2s, see table below, 20µM)................................20 µl each
• 10X Human Ubiquitin (500 µM)................................200 µl
• 10X Ubiquitination Buffer.............................................1 ml
• 40 mM ATP...............................................................250 µl

E2 Name

1: 6XHis-Ube2A (Rad6A)
2: 6XHis-Ube2B
3: 6XHis-Ube2C(UbcH10 )
4: 6XHis-Ube2D1 (UbcH5A)
5: 6XHis-Ube2D2 (UbcH5B)
6: 6XHis-Ube2D3 (UbcH5C)
7: 6XHis-Ube2D4 (UbcH5D)
8: 6XHis-Ube2E1 (UbcH6)
9: 6XHis-Ube2E2 (UbcH8)
10: 6XHis-Ube2E3 (UbcH9)
11: 6XHis-Ube2F
12: 6XHis-Ube2G1
13: 6XHis-Ube2G2
14: 6XHis-Ube2H (UbcH2)
15: 6XHis-Ube2I
16: 6XHis-Ube2J1(1-282)
17: GST-Ube2K (E2-25K)
18: 6XHis-Ube2L3
19: 6XHis-Ube2L6
20: 6XHis-Ube2M (UbcH12)
21: 6XHis-UbeN (Ubc13/Uev1a)
22: 6XHis-Ube2Q2
23: 6XHis-Ube2R1 (Cdc34)
24: 6XHis-Ube2R2
25: 6XHis-Ube2S
26: 6XHis-Ube2T
27: 6xHis-Ubc13/UbE2V2
28: 6XHis-Ube2W
29: 6XHis-Ube2Z