Deubiquitinating Enzyme Inhibitor Profiling Kit I

This kit is designed to determine DUB inhibitor specificity and potency. You can select 10 DUBs of your choice from our DUB list. Each DUB is sufficient for 20 X 50ul reactions using a working concentration of 10nM. The supplied Ub-rhodamine 110 is sufficient for 550 X 50ul reactions using a working concentration of 100nM. The supplied iodoacetamide can be used as an inhibitor of cysteine DUBs using a working concentration of 10mM.

Additional Information

Product Name: Deubiquitinating Enzyme Inhibitor Profiling Kit I
Also Known As: N/A
Catalog No.: J6110
Size: 1 Kit
Molecular Weight: N/A
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: 90% - 95% based on SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet
NCBI RefSeq: N/A
Image(s): N/A
Shipping Method: Dry ice shipping
References: N/A

Details

* When place an order, please select any ten (10) DUBs from the list on the right side of this page.

Description

This kit is designed to determine DUB inhibitor specificity and potency. You can select 10 DUBs of your choice from our DUB list. Each DUB is sufficient for 20 X 50ul reactions using a working concentration of 10nM. The supplied Ub-rhodamine 110 is sufficient for 550 X 50ul reactions using a working concentration of 100nM. The supplied iodoacetamide can be used as an inhibitor of cysteine DUBs using a working concentration of 10mM.

Components

• 10X DUBs (pick 10 DUBs from the list, 200nM)..................50 µl each
• Ub-rhodamine 110 (1mg/ml)............................................................25 µl
• Iodoacetamine (IAA).........................................................................25 mg
• 5X Deubiquitination Buffer.........................................................2X1.25ml

DUBs List
1. 6xHis-Uch37
2. Adrm1/Uch37
3. 6xHis-Usp14
4. UCHL1
5. GST-UCHL1
6. UCHL3
7. GST-UCHL3
8. GST-Ataxin3(Q22)
9. OTUB1
10. GST-OTUB1
11. OTUB2
12. GST-OTUB2
13. GST-A20(1-365)
14. OTUD2
15. OTUD6B
16. 6xHis-OTULIN
17. GST-USP4
18. USP7
19. GST-USP7
20. GST-USP9X (catalytic domain)
21. GST-USP11
22. GST-USP15
23. GST-USP21(195-565)
24. GST-USP30(57-517)